Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

This Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit.

Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated human IGF-IRα subunit in 0.5 mg of human IGF-IR-expressing C4 cell lysate.
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected IGF-IRα subunit in human IGF-IR-expressing C4 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Insulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor has tyrosine kinase that is activated by IGF-1 and IGF-2 binding. IGF-1 receptor is initially produced as a precursor protein with an aignal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine auto-phosphorylation of the beta chains, which in turn triggers cell type-specific downstream signaling cascade of events.

Immunogen

KLH-conjugated synthetic peptide [C-KYADGTIDIEEVTENPKT] corresponding to amino acid residues 672-689 of human IGF-IR alpha chain.

Epitope: Third Fibronectin Type III (FnIII 3) domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified chicken polyclonal IgY antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected IGF-IRα subunit in 10 µg of human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~110/180 kDa observed